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KMID : 0603820100160040299
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Journal of Experimental & Biomedical Science
2010 Volume.16 No. 4 p.299 ~ p.305
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Cloning and Expression of Human KCNE1 Gene
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Ye Qing
Kim Su-Won
Kim Jong-Won
Yoo Min
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Abstract
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KCNE1 is the causal gene of long QT syndrome. KCNE1 gene is located in chromosome 21. In compliance with this KCNE1 gene the proteins come out. KCNE1 is responsible for K? channel which maintains the normal function of the heart muscle for contraction. Affected individuals manifest prolongation of the QT interval on electrocardiongrams, a sign of abnormal cardiac repolarization. The clinical features of LQT result from episodic cardiac arrhythmias, such as torsade de pointes and ventricular fibrllation. Blood DNA was isolated and kept in 4℃ refrigerator. The KCNE1 gene was amplified by PCR method and about 414 bp band was identified by agarose gel electrophoresis. PCR products were inserted into pGEX-4T-1 vector in order to express KCNE1 protein after treatment with IPTG SDS-PAGE was carried out and the protein band which was about 47 kDa was clearly odserved. Results of this study would contribute to the detailed understanding of KCNE1 protein function and to designing better treatment of Long QT symdrome.
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KEYWORD
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KCNE1 geng, Long QT syndrome, QT interval, K⁺ channel, pGEX-4T-1 vector, SDS-PAGE, Clonging, Expression
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